Cell Cycle Checkpoint Control Protocols (Methods in Molecular Biology (Clifton, N.J.), V. 241.)
Product Details
»Book Publisher: Humana Press (November, 2003)
»ISBN: 1588291154
»Book author: Howard B. Lieberman
Book Description:
A collection of basic cutting-edge techniques for studying the mechanisms underlying cell cycle regulation and checkpoint control. Using mammalian, yeast, and frog systems, these readily reproducible methods can be used to induce cell cycle checkpoints, detect changes in cell cycle progression, identify and analyze genes and proteins that regulate the process, and characterize chromosomal status as a function of cell cycle phase and progression. Each fully tested technique includes step-by-step instructions written by an investigator who routinely performs it, an introduction explaining the principle behind the method, equipment and reagent lists, and tips on troubleshooting and avoiding known pitfalls.
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Ribozyme Protocols (Methods in Molecular Biology)
Product Details
»Book Publisher: Humana Press (15 June, 1997)
»ISBN: 0896033899
»Book author: P. C. Turner
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Kinesin Protocols (Methods in Molecular Biology)
Product Details
»Book Publisher: Humana Press (15 January, 2001)
»ISBN: 0896037665
»Book author: Isabelle Vernos
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Mitochondria
Product Details
»Book Publisher: Wiley-Liss (05 July, 1999)
»ISBN: 0471194220
»Book author: Immo E. Scheffler
Book Description:
Mitochondria are complex organelles, possessing inner and outer membranes and even their own DNA. A typical cell contains 800 mitochondria, termed the “powerhouse of the cell”, playing a central role in metabolism, respiration, and ATF production. Mitochondria is the first modern, truly comprehensive authored book which tackles the biochemistry, genetics, and pathology of mitochondria in different organisms.
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Macromolecular Crystallography Protocols: Preparation And Crystallization of Macromolecules (Methods in Molecular Biology)
Macromolecular Crystallography Protocols: Preparation And Crystallization of Macromolecules (Methods in Molecular Biology)
Product Details
»Book Publisher: Humana Press (15 January, 2007)
»ISBN: 1588292924
»Book author: Sylvie Doublie
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Cell Motility: From Molecules to Organisms (Life Sciences)
Product Details
»Book Publisher: John Wiley & Sons (17 March, 2004)
»ISBN: 0470848723
»Book author: Anne Ridley, Michelle Peckham, Peter Clark
»Amazon Rating:
Book Description:
Recent advances in molecular and biophysical techniques, particularly fluorescence and live cell imaging, are revolutionizing the study of cell motility. New bioprobes not only reveal simple intracellular localization, but also contain details of post-translational modifications, conformational state and protein-protein interactions. Coupling these insights with complementary advances in genetic and biochemical methods is enabling scientists to understand the processes involved in cell motility - from molecular motors to cell movements in vivo in a range of organisms and cell types.
This book features landmark essays that provide an up to date and fascinating account of current research and concepts in cell motility.These cover the roles of molecular motors that drive movement and their interactions with the cytoskeleton as well as membrane dynamics that allow cells to change shape and to move.
Cell motility plays a key role in development - there are chapters on the genetics of cell migration, the regulation of contact repulsion in growth cones, and the progression from cell migration to cell-cell adhesion. Cell motility is directional - experts describe the molecules that regulate chemotaxis, allowing cells to migrate along pathways specified by chemical gradients. Finally, cell motility can be perturbed by mutation–metastasis occurs when cells lose their normal intercellular interactions and invade other tissue types. All these processes are regulated by signals from the environment, including other tissues in the body, and the various molecules that transmit and transduce these signals are discussed.
This book is a ‘must read’ for cell biologists working in a variety of fields, from development to wound healing, at all levels - post-doctoral fellows, post-graduate students and lab technicians. It is also stimulating reading for molecular and developmental biologists, biophysicists and biochemists.
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Transmembrane Transporters (Receptor Biochemistry and Methodology)
Product Details
»Book Publisher: Wiley-Liss (09 September, 2002)
»ISBN: 0471065137
»Book author: Michael W. Quick
»Amazon Rating:
Book Description:
A must-have far-reaching text that provides readers with a state-of-the-art molecule update on transmembrane transporters, focusing on the methodological approaches currently employed to better understand how transporters work and how they can be used in cutting edge therapies. Each chapter begins with an overview of the importnat biological questions presently being considered in their field, then presents scientific approaches to address these questions. In explaining approaches, the authors cover bench-top protocols, conceptual frameworks, data obtained, and pitfalls common to the techniques.
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Cell Engineering: Apoptosis (Cell Engineering)
Product Details
»Book Publisher: Springer (03 August, 2004)
»ISBN: 1402022166
»Book author: Mohamed Al-Rubeai, Martin Fussenegger
Book Description:
The series Cell Engineering is the first and only major reference work on the development of cellular systems for the production of recombinant glycoproteins, gene and cell therapies, drug screening and tissue engineering. This volume on “Apoptosis” is intended to review the state-of-the-art with in-depth assessments of this type of programmed cell death. The aim of the volume is to make the recent developments in apoptotic research readily accessible to biologists, biotechnologists and cellular engineers. The implication of apoptosis in the suppression of diseases and prolonging survival of cells in culture is presented to indicate the great potential of apoptotic research for drug production and the development of human therapies. All chapters are written as self-contained treatments of the important topics in apoptosis that are presented on an essential information basis. Topics covered range from understanding the role of signalling and effector molecules, mathematical modelling of cell death, RNAi tools in apoptosis research, to monitoring and imaging of apoptosis. This volume will be an invaluable resource for biotechnologists and researchers in apoptosis, cell biology, cell culture and molecular medicine.
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Molecular Evolution: Producing the Biochemical Data, Volume 224: Volume 224: Molecular Evolution (Methods in Enzymology)
Molecular Evolution: Producing the Biochemical Data, Volume 224: Volume 224: Molecular Evolution (Methods in Enzymology)
Product Details
»Book Publisher: Academic Press (12 October, 1993)
»ISBN: 0121821250
»Book author: John N. Abelson, Melvin I. Simon, Elizabeth A. Zimmer, Thomas J. White, Rebecca L. Cann, Allan C. Wilson
Book Description:
This book provides a single source of information for all molecular methods. Expert advice is given on sample collection and storage for molecular work.
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Methods in Enzymology, Volume 356: Laser Capture in Microscopy and Microdissection (Methods in Enzymology)
Methods in Enzymology, Volume 356: Laser Capture in Microscopy and Microdissection (Methods in Enzymology)
Product Details
»Book Publisher: Academic Press (October, 2002)
»ISBN: 0121822591
»Book author: P. Michael Conn
Book Description:
The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today—truly an essential publication for researchers in all fields of life sciences.
Major Sections Include:
* Basic Principles, Specialized Uses, and Genetic Applications
* LCM and its application in genomics and proteomics * Fluorescence in situ hybridization of LCM isolated nuclei from paraffin sections
* Noncontact laser catapulting for the functional genomics and proteomics
* Use of LCM for clonal analysis, in carcinoma analysis, to assess development, in complex tissue, in pathology, gene discovery, and more.
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Nuclear Receptors, Volume 364 (Methods in Enzymology)
Product Details
»Book Publisher: Academic Press (10 November, 2003)
»ISBN: 0121822672
»Book author: David W. Russell
»Amazon Rating: 4.0
Book Description:
Nuclear Receptors are inducible transcription factors that mediate complex effects on development, differentiation and homeostasis. They regulate the transcription of their target genes through binding to DNA sequences.
*Analysis of Nuclear Receptor Ligands
*Structure/Function Analysis of Nuclear Receptors
*Analysis of Nuclear Receptor Co-Factors and Chromatin Remodeling
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Protein NMR Techniques (Methods in Molecular Biology)
Product Details
»Book Publisher: Humana Press (15 August, 1997)
»ISBN: 0896033090
»Book author: David G. Reid
Book Description:
SmithKline Beecham Pharmaceuticals, Hertfordshire, U.K. Text on how to apply the fundamentals of NMR to the study of proteins. For chemists and biochemists. 29 contributors, 26 U.S.
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Protein Targeting Protocols (Methods in Molecular Biology)
Product Details
»Book Publisher: Humana Press (June, 1998)
»ISBN: 089603450X
»Book author: Roger A. Clegg
»Amazon Rating:
Book Description:
Hannah Research Institute, Scotland, UK. Text of methods for detecting and analyzing the proteins and protein motifs involved in cellular targeting interactions. Modified-outline format. Plasticcomb binding, hardcover available.
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RNA Polymerase and Associated Factors, Part D, Volume 371 (Methods in Enzymology)
Product Details
»Book Publisher: Academic Press (16 December, 2003)
»ISBN: 0121822745
»Book author: Sankar Adhya, Susan Garges
Book Description:
RNA polymerase is molecule important to gene transcription. Along with associated factors, RNA polymerase is part of the process in which RNA is transcribed to produce a protein.
* Models and methods for studying polymerase translocation
* Assay for movements of RNA polymerase along DNA
* Engineering of elongation complexes of bacterial and yeast RNA polymerases
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Signal Transduction
Product Details
»Book Publisher: Academic Press (March, 2002)
»ISBN: 0122896319
»Book author: Bastien D. Gomperts, Ijsbrand M. Kramer, Peter E.R. Tatham
»Amazon Rating: 4.0
Book Description:
Signal Transduction is a well-illustrated, coherent look at cellular signaling processes. Beginning with the basics, it explains how cells respond to external cues, hormones, growth factors, cytokines, cell surfaces, etc., and then shows how these inputs are integrated and coordinated. The extensive color artwork provides the reader with a clearer comprehension of key topics, and margin notes are incorporated to highlight milestones in the evolution of signal transduction. This book serves as an invaluable resource for advanced undergraduates, graduate researchers, and established scientists working in cell biology, pharmacology, immunology, and related fields.
Key Features
* Coherent, precise text providing insight in depth to a subject that is central to cell biology and fundamental to many areas of biomedicine
* Extensive conceptual colour artwork assists with the comprehension of key topics
* Extensive referencing provides an invaluable link to the core and historical literature
* Margin notes highlighting milestones in the evolution of our understanding of signalling mechanisms
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RNA Polymerase and Associated Factors, Part C, Volume 370 (Methods in Enzymology)
Product Details
»Book Publisher: Academic Press (16 December, 2003)
»ISBN: 0121822737
»Book author: Sankar Adhya, Susan Garges
»Amazon Rating:
Book Description:
RNA polymerase is molecule important to gene transcription. Along with associated factors, RNA polymerase is part of the process in which RNA is transcribed to produce a protein.
* Construction and purification of RNA polymerases
* DNA microarrays and bacterial gene expression
* Functional analysis of transcription factors
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Liposomes, Part C, Volume 373 (Methods in Enzymology)
Product Details
»Book Publisher: Academic Press (19 December, 2003)
»ISBN: 0121822761
»Book author: Nejat Duzgunes
Book Description:
Liposomes are cellular structures made up of lipid molecules. Important as a cellular model in the study of basic biology liposomes are also used in clinical applications such as drug delivery and virus studies.
*Liposomes in Immunology
*Liposomes in Diagnostics
*Liposomes in Gene Delivery and Gene Therapy
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Chromosomal Instability and Aging
Product Details
»Book Publisher: Informa Healthcare (February, 2003)
»ISBN: 0824708563
»Book author: Fuki Hisama, Sherman M. Weissman, George M. Martin
Book Description:
This text examines the relationship between DNA damage and repair, cellular senescence, genomic instability and ageing. It includes in-depth discussions of various types of DNA damage, the DNA repair network, and cellular responses to genetic damage to assess their impact on the modulation of ageing processes and age-related disease, including cancer development.
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Sodium Channels and Neuronal Hyperexcitability
Product Details
»Book Publisher: John Wiley & Sons (15 December, 2001)
»ISBN: 0471485306
»Book author: Novartis Foundation
Book Description:
Recent progress has led to a better understanding of the molecular structure of sodium channels, how they work and the mechanisms that restrict their expression to particular cell types.
This book explains how these channels are modulated in various pathological states to enable the development of therapeutic strategies based on sodium channel restoration.
The authors present the most current research on sodium channels and
neuronal function.
- Brings together both the basic biology and biophysics with detailed consideration of the medical applications in chronic pain and epilepsy.
- Includes coverage of channelopathies, sodium channel gene expression, the modulation of sodium channels, and molecular mechanisms of gating and drug block of sodium channels.
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DNA Microarrays, Part B: Databases and Statistics, Volume 411 (Methods in Enzymology)
Product Details
»Book Publisher: Academic Press (28 August, 2006)
»ISBN: 0121828166
»Book author: Alan R. Kimmel, Brian Oliver
Book Description:
Modern DNA microarray technologies have evolved over the past 25 years to the point where it is now possible to take many million measurements from a single experiment. These two volumes, Parts A & B in the Methods in Enzymology series provide methods that will shepard any molecular biologist through the process of planning, performing, and publishing microarray results.
Part A starts with an overview of a number of microarray platforms, both commercial and academically produced and includes wet bench protocols for performing traditional expression analysis and derivative techniques such as detection of transcription factor occupancy and chromatin status. Wet-bench protocols and troubleshooting techniques continue into Part B. These techniques are well rooted in traditional molecular biology and while they require traditional care, a researcher that can reproducibly generate beautiful Northern or Southern blots should have no difficulty generating beautiful array hybridizations.
Data management is a more recent problem for most biologists. The bulk of Part B provides a range of techniques for data handling. This includes critical issues, from normalization within and between arrays, to uploading your results to the public repositories for array data, and how to integrate data from multiple sources. There are chapters in Part B for both the debutant and the expert bioinformatician.
Provides an overview of platforms
Includes experimental design and wet bench protocols
Presents statistical and data analysis methods, array databases, data visualization and meta analysis
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