Product Details
»Book Publisher: Springer (12 January, 2005)
»ISBN: 3540220062
»Book author: Michael R. Emmert-Buck, John W. Gillespie, Rodrigo F. Chuaqui
»Amazon Rating:
Labels: anatomy, Biology, Immunology, Molecular Biology, Pathology
Calpain Methods and Protocols (Methods in Molecular Biology)
Product Details
»Book Publisher: Humana Press (March, 2000)
»ISBN: 0896036324
»Book author: John S. Elce
Book Description:
Queen’s Univ. Kingston, Ontario, Canada. Set of proven and easily followed protocols for studying calpain. Detailed methods to investigate the physiological and pathological roles of calpain. A wide range of calpain systems and tissue models. Specific lists Specific lists of all required materials and notes on pitfalls.
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Labels: Biochemistry, Molecular Biology
Inflammation Protocols (Methods in Molecular Biology, V. 225)
Product Details
»Book Publisher: Humana Press (April, 2003)
»ISBN: 0896039706
»Book author: Paul G. Winyard, D. A. Willoughby
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In Situ Hybridization Protocols (Methods in Molecular Biology)
Product Details
»Book Publisher: Humana Press (September, 1994)
»ISBN: 0896032809
»Book author: K. H. Andy Choo
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Labels: Biology, Laboratory, Molecular Biology
Embryonic Stem Cell Protocols: Differentiation Models (Methods in Molecular Biology)
Product Details
»Book Publisher: Humana Press (20 February, 2006)
»ISBN: 1588297845
»Book author: Kursad Turksen
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Labels: Embryology, Molecular Biology
Molecular Embryology: Methods and Protocols (Methods in Molecular Biology)
Product Details
»Book Publisher: Humana Press (15 January, 1999)
»ISBN: 0896033872
»Book author: Paul T. Sharpe, Ivor Mason
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Labels: Biology, Embryology, Molecular Biology
Color Atlas of Histopathology of the Cervix Uteri
Product Details
»Book Publisher: Springer (26 October, 2005)
»ISBN: 354025188X
»Book author: Gisela Dallenbach-Hellweg, Magnus von Knebel Doeberitz, Marcus J. Trunk
Book Description:
The second edition of this atlas provides a detailed histopathological diagnosis of cervical disease emphasizing carcinogenesis, and correlating morphologic changes with clinical aspects. Instructions of how to obtain and handle tissue samples are followed by a description of the normal histology, of the action of steroid hormones on the cervix, and of the various forms of regeneration and inflammation. The section on etiology and pathogenesis of HPV-induced neoplasia has been enlarged to explain new insights into cellular and nuclear deregulation at the molecular level. A chapter on immunohistochemistry has been added which includes refined detection methods, e.g., with p16 for the early differential diagnosis of premalignant lesions. The latest advances in molecular biological research, with predictive subdivision of neoplasias, facilitate the choice of the proper therapy. All chapters – supported by over 20 new microphotographs – have been revised to include advances in the interpretation and management of cervical disease. The tumor nomenclature is adapted to the new WHO classification; the ICD-O code has been added. The reference list is updated with relevant publications.
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Labels: Gynecology, Molecular Biology, Obstetrics, Oncology, Pathology
Calcium-Binding Protein Protocols: Volume 1: Reviews and Case Histories (Methods in Molecular Biology)
Calcium-Binding Protein Protocols: Volume 1: Reviews and Case Histories (Methods in Molecular Biology)
Product Details
»Book Publisher: Humana Press (15 December, 2001)
»ISBN: 089603688X
»Book author: Hans J. Vogel
Book Description:
Blurb for Volume 1 Hans Vogel and a panel of leading researchers review the protein chemistry and behavior of this significant protein class, and provide a comprehensive collection of proven experimental techniques for their study both in vitro and in vivo. This first volume discusses the role of calcium in intracellular secondary messenger activation mechanisms, including unique aspects of calcium chemistry and its utilization in diary proteins and blood clotting. Detailed case studies provide a wealth of valuable information about protein purification and characterization strategies, X-ray crystallography, and specific calcium-binding proteins and their modes of action. The second companion volume focuses on cutting-edge experimental methods for studying solution structure, stability, dynamics, calcium-binding properties, and biological activity of calcium-binding proteins in general. Single Blurb for Both Volumes: Hans Vogel and a panel of leading researchers review the protein chemistry and behavior of this significant protein class, and provide a comprehensive collection of proven experimental techniques for their study both in vitro and in vivo. The first volume discusses the role of calcium in intracellular secondary messenger activation mechanisms, including unique aspects of calcium chemistry and its utilization in dairy proteins and blood clotting. Detailed case studies provide a wealth of valuable information about protein purification and characterization strategies, X-ray crystallography, and specific calcium-binding proteins and their modes of action. The second volume focuses on cutting–edge experimental techniques for studying the solution structure, stability, dynamics, calcium-binding properties, and biological activity of calcium-binding protein in general. In addition to enzymatic assays and more routine spectroscopic and protein chemistry techniques, there are also NMR approaches, thermodynamic analyses, kinetic measurements such as surface plasmon resonance, strategies for amino acid sequence alignments, and fluorescence methods to study the distribution of calcium and calcium–binding proteins in cells.
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Labels: Biochemistry, Molecular Biology
The ELISA Guidebook (Methods in Molecular Biology)
Product Details
»Book Publisher: Humana Press (August, 2000)
»ISBN: 0896037282
»Book author: John R. Crowther
Book Description:
John R. Crowther provides today’s premier practical guide to the understanding and application of ELISA. Updating and greatly expanding his widely appreciated earlier publication, ELISA Theory and Practice (1995), this important work introduces chapters on such major new topics as checkerboard titrations, quality control of testing, kit production and control, novel monoclonal antibodies, validation of assays, statistical requirements for data examination, and epidemiological considerations. With its numerous worked examples, detailed instructions, and extensive illustrations, The ELISA Guidebook offers a powerful synthesis of all the basic concepts and practical experimental details investigators need to understand, develop, and apply the new ELISA methodology successfully in day-to-day basic and clinical research.
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Labels: Immunology, Laboratory, Molecular Biology
Cancer Cytogenetics: Methods and Protocols (Methods in Molecular Biology, 220)
Product Details
»Book Publisher: Humana Press (April, 2003)
»ISBN: 1588290808
»Book author: John Swansbury
»Amazon Rating:
Book Description:
The Royal Marsden NHS Trust, Surrey, UK. Text describes in detail the key cytogenetic and FISH techniques used in the genetic analysis of human malignancies and how to interpret the results. Each protocol contains novel ideas and time-saving techniques. Also provides guidance on the significance and interpretation of findings.
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Labels: Genetics, Molecular Biology, Oncology, Pathology
Cytokine Reference: A Compendium of Cytokines and Other Mediators of Host Defense (Institutional Version)
Cytokine Reference: A Compendium of Cytokines and Other Mediators of Host Defense (Institutional Version)
Product Details
»Book Publisher: Academic Press (September, 2000)
»ISBN: 0122526732
»Book author: Joost J. Oppenheim, Marc Feldman
Book Description:
The Cytokine Reference is the most complete work for anyone interested in the role of cytokines in host defense processes. With essential and comprehensive information on all known cytokines, including chemokines, growth factors and neuropeptides, the database will include hundreds of entries with detailed descriptions of cytokine genes, proteins, cell sources, activities, receptors, receptor signal transduction, gene activation, pathophysiology, therapeutics and techniques. This encyclopedic reference is written by a panel of global experts and will include extensive tables and diagrams. This book/online bundle consists of a two-volume print set, plus a regularly updated and searchable Online Database.
Cytokines are produced by virtually all the cells in our bodies, and they regulate the systems that defend us against infection, tumors, and trauma. This publication and database will enable all those working in the field to find the information they need about this ever-expanding field.
Key Features
* Online extensively-linked database is highly functional and allows you to reach data in a variety of ways–by cytokine, by cell type, or by disease
* Online version offers regularly updated entries and addition of new entries as research uncovers further information
* Print version has approximately 2500 pages of detailed and highly structured information on all the cytokines and other mediators of host defense
* Contains over 175 chapters contributed by international experts and edited by 6 world renowned scientists
* Online database allows extensive links at chapter subheading level guiding you through the maze of inter-relationships
* Online version includes full color diagrams and sequences plus links to online databases, including MEDLINE, PDB, SWISSPRO, and GenBank
* Every cytokine or receptor chapter uses the same highly structured subheadings, ensuring that the information is complete, comprehensive, and easy to find
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Cell Cycle Checkpoint Control Protocols (Methods in Molecular Biology (Clifton, N.J.), V. 241.)
Product Details
»Book Publisher: Humana Press (November, 2003)
»ISBN: 1588291154
»Book author: Howard B. Lieberman
Book Description:
A collection of basic cutting-edge techniques for studying the mechanisms underlying cell cycle regulation and checkpoint control. Using mammalian, yeast, and frog systems, these readily reproducible methods can be used to induce cell cycle checkpoints, detect changes in cell cycle progression, identify and analyze genes and proteins that regulate the process, and characterize chromosomal status as a function of cell cycle phase and progression. Each fully tested technique includes step-by-step instructions written by an investigator who routinely performs it, an introduction explaining the principle behind the method, equipment and reagent lists, and tips on troubleshooting and avoiding known pitfalls.
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Labels: Cytology, Molecular Biology
Calcium-Binding Protein Protocols: Volume 2: Methods and Techniques (Methods in Molecular Biology)
Product Details
»Book Publisher: Humana Press (15 December, 2001)
»ISBN: 0896036898
»Book author: Hans J. Vogel
Book Description:
Blurb for Volume 2 Hans Vogel and a panel of leading researchers review the protein chemistry and behavior of this significant class proteins, and provide a comprehensive collection of proven experimental techniques for studying it both in vitro and in vivo. This second volume focuses on cutting–edge experimental techniques for studying the solution structure, stability, dynamics, calcium-binding properties, and biological activity of calcium-binding protein in general. In addition to enzymatic assays and more routine spectroscopic and protein chemistry techniques, there are also NMR approaches, thermodynamic analyses, kinetic measurements such as surface plasmon resonance, strategies for amino acid sequence alignments, and fluorescence methods to study the distribution of calcium and calcium–binding proteins in cells. The first companion volume, Reviews and Case Histories sets the stage for this volume by introducing the various classes of intra- and extra-cellular calcium-binding proteins and their mode of action. Single Blurb for Both Volumes Hans Vogel and a panel of leading researchers review the protein chemistry and behavior of this significant protein class, and provide a comprehensive collection of proven experimental techniques for their study both in vitro and in vivo. The first volume discusses the role of calcium in intracellular secondary messenger activation mechanisms, including unique aspects of calcium chemistry and its utilization in dairy proteins and blood clotting. Detailed case studies provide a wealth of valuable information about protein purification and characterization strategies, X-ray crystallography, and specific calcium-binding proteins and their modes of action. The second volume focuses on cutting–edge experimental techniques for studying the solution structure, stability, dynamics, calcium-binding properties, and biological activity of calcium-binding protein in general. In addition to enzymatic assays and more routine spectroscopic and protein chemistry techniques, there are also NMR approaches, thermodynamic analyses, kinetic measurements such as surface plasmon resonance, strategies for amino acid sequence alignments, and fluorescence methods to study the distribution of calcium and calcium–binding proteins in cells.
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Labels: Molecular Biology
Ribozyme Protocols (Methods in Molecular Biology)
Product Details
»Book Publisher: Humana Press (15 June, 1997)
»ISBN: 0896033899
»Book author: P. C. Turner
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Labels: Cytology, Genetics, Laboratory, Molecular Biology
Methods in Enzymology, Volume 302: Green Flourescent Protein (Methods in Enzymology)
Product Details
»Book Publisher: Academic Press (15 April, 1999)
»ISBN: 0121822036
»Book author: John N. Abelson, Melvin I. Simon, P. Michael Conn
»Amazon Rating: 5.0
Book Description:
One of the most important recent discoveries in science is the green fluorescent protein, isolated from a bioluminescent jellyfish. The gene that codes for this protein is active in any type of cell, from microbes to humans, producing a “day-glo” dye visible as a bright green light in the microscope. The power of the gene is that by using recombinant DNA technology. GFP can be spliced to any protein, essentially painting that protein green. When such a spliced protein is inside a cell. Further, time-lapse microscopy can be used to examine how that protein and the cellular components that it is part of move within the cell during the cell’s life cycle.
This Methods in Enzymology volume deals with the utility of green fluorescent protein (GFP). The OVID database (including MEDLINE, Current Contents, and other sources) lists nine references to GFP for the ten-year period 1985-1994. In contrast, in less than four years thereafter, over 500 references are listed, a testament to the rapid growth of interest in this probe.
This volume documents many diverse uses for this interesting molecule in disciplines that broadly span biology. The methods presented include shortcuts and conveniences not included in previously published sources. The techniques are described in a context that allows comparisons to other related methodologies-such comparisons are valuable to readers who must adapt existing procedures to new systems. Also, so far as possible, methodologies have been presented in a manner that stresses their general applicability and potential limitations. The volume provides a substantial and current overview of the extant methodology in the field and a view of its rapid development.
The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today–truly an essential publication for researchers in all fields of life sciences.
Key Features
* Monitoring of Physiological Processes
* Localization of Molecules
* Special Uses
* Mutants
* Variants of GFP
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Labels: Biology, Molecular Biology
Kinesin Protocols (Methods in Molecular Biology)
Product Details
»Book Publisher: Humana Press (15 January, 2001)
»ISBN: 0896037665
»Book author: Isabelle Vernos
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Labels: Biochemistry, Biology, Cytology, Genetics, Laboratory, Molecular Biology
Mitochondria
Product Details
»Book Publisher: Wiley-Liss (05 July, 1999)
»ISBN: 0471194220
»Book author: Immo E. Scheffler
Book Description:
Mitochondria are complex organelles, possessing inner and outer membranes and even their own DNA. A typical cell contains 800 mitochondria, termed the “powerhouse of the cell”, playing a central role in metabolism, respiration, and ATF production. Mitochondria is the first modern, truly comprehensive authored book which tackles the biochemistry, genetics, and pathology of mitochondria in different organisms.
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Labels: Cytology, Genetics, Molecular Biology
Consciousness In Four Dimensions: Biological Relativity and the Origins of Thought
Product Details
»Book Publisher: McGraw-Hill Companies (17 October, 2001)
»ISBN: 0071354999
»Book author: Richard M., Ph.D. Pico
»Amazon Rating: 5.0
Book Description:
In a book that will profoundly alter the modern discourse on mind and influence the practice of neuromedicine, neurobiologist/neuropsychiatrist, Richard M. Pico unveils a revolutionary new approach to understanding consciousness that pinpoints its origins in the brain. Called Biological Relativity, the approach combines the laws of physics–especially Einstein’s laws of relativity–to the latest breakthroughs in neuroscience, molecular biology, and computational theory to create a coherent four-dimensional model for explaining the origins of life and the emergence of complex biological systems–from the living cell to the thinking brain.
In a fascinating, ambitious narrative that draws upon a lifetime of experimental and clinical work, Dr. Pico tells a riveting story that begins in the imponderably distant past, with the first proto-cell that endured long enough to become its own frame of reference–both structurally and temporally–and culminates with the most complex biological referent system known to science, the human brain. He then elaborates his groundbreaking theory through discussions of such things as the origins of language, music, and mathematics. He explains why he believes consciousness is uniquely human, and explores the causes and potential treatments for a variety of thought disorders.
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Labels: Molecular Biology, Neuroscience
Macromolecular Crystallography Protocols: Preparation And Crystallization of Macromolecules (Methods in Molecular Biology)
Macromolecular Crystallography Protocols: Preparation And Crystallization of Macromolecules (Methods in Molecular Biology)
Product Details
»Book Publisher: Humana Press (15 January, 2007)
»ISBN: 1588292924
»Book author: Sylvie Doublie
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Labels: Cytology, Molecular Biology
Molecular Biology Problem Solver: A Laboratory Guide
Product Details
»Book Publisher: Wiley-Liss (24 August, 2001)
»ISBN: 0471379727
»Book author: Alan S. Gerstein
»Amazon Rating:
Book Description:
Most research in the life sciences involves a core set of molecular-based equipment and methods, for which there is no shortage of step-by-step protocols. Nonetheless, there remains an exceedingly high number of inquiries placed to commercial technical support groups, especially regarding problems.
Molecular Biology Problem Solver: A Laboratory Guide asks the reader to consider crucial questions, such as:
- Have you selected the most appropriate research strategy?
- Have you identified the issues critical to your successful application of a technique?
- Are you familiar with the limitations of a given technique?
- When should common procedural rules of thumb not be applied?
- What strategies could you apply to resolve a problem?
A unique question-based format reviews common assumptions and laboratory practices, with the aim of offering a firm understanding of how techniques and procedures work, as well as how to avoid problems. Some major issues explored by the book’s expert contributors include:
- Working safely with biological samples and radioactive materials
- DNA and RNA purification
- PCR
- Protein and nucleid acid hybridization
- Prokaryotic and eukaryotic expression systems
- Properly using and maintaining laboratory equipment
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Labels: Biology, Laboratory, Molecular Biology